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microrna microarray data analysis  (LC Sciences)

 
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    LC Sciences microrna microarray data analysis
    Microrna Microarray Data Analysis, supplied by LC Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microrna+microarray+analysis/mirna+microarray/us12311034-426-0-5
    Average 90 stars, based on 1 article reviews
    microrna microarray data analysis - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: Micro-RNA30c Negatively Regulates REDD1 Expression in Human Hematopoietic and Osteoblast Cells after Gamma-Irradiation
    Article Snippet: RNA concentrations were determined by measuring OD on the NanoDrop spectrophotometer ND-1000 (Thermo Fisher Scientific) and total RNA quality was verified on the Agilent 2100 bioanalyzer (Agilent Technologies) with RNA 6000 Nano chips. .. MicroRNA microarray analysis was performed by LC Sciences (Houston, Texas) on unirradiated or 2 or 8 Gy γ-irradiated human CD34+ and hFOB cells collected 1 h after irradiation. ..

    Article Title: Microarray-Based Approach Identifies Differentially Expressed MicroRNAs in Porcine Sexually Immature and Mature Testes
    Article Snippet: .. MicroRNA microarray analysis was performed by LC Sciences (Houston, TX). .. Briefly, the assay started from 2 to 5 μg total RNA sample, which was size fractionated using a YM-100 Microcon centrifugal filter (from Millipore) and the small RNAs (<300 nt) isolated were 3′-extended with a poly(A) tail using poly(A) polymerase.

    Article Title: Mutant p53-R273H gains new function in sustained activation of EGFR signaling via suppressing miR-27a expression
    Article Snippet: H1299-Tet-On-p53 and H1299-Tet-On-p53 273H cells were treated with or without doxycycline for 24 h. Total RNA from these cells was isolated with TRIzol reagent (Invitrogen, Grand Island, NY, USA). .. To compare the miRNA expression profiles between wild-type p53- and mutant p53-expressing H1299 cells, the μParaflo MicroRNA microarray analysis was performed by LC Sciences (Houston, TX, USA). ..

    Article Title: CD97 promotes gastric cancer cell proliferation and invasion through exosome-mediated MAPK signaling pathway
    Article Snippet: MicroRNA microarray assay Total RNA from exosomes was isolated using the miRNeasy Mini Kit (Qiagen, Germany). .. MicroRNA microarray analysis following the miRbase v20.0 was performed by LC Sciences (Hangzhou, China; http://www.lc-bio.com/ ). ..

    Article Title: Micro-RNA30c negatively regulates REDD1 expression in human hematopoietic and osteoblast cells after gamma-irradiation.
    Article Snippet: RNA concentrations were determined by measuring OD on the NanoDrop spectrophotometer ND-1000 (Thermo Fisher Scientific) and total RNA quality was verified on the Agilent 2100 bioanalyzer (Agilent Technologies) with RNA 6000 Nano chips. .. MicroRNA microarray analysis was performed by LC Sciences (Houston, Texas) on unirradiated or 2 or 8 Gy c-irradiated human CD34+ and hFOB cells collected 1 h after irradiation. ..

    Article Title: Immunomodulation by Bifidobacterium animalis subsp. lactis Bb12: Integrative Analysis of miRNA Expression and TLR2 Pathway–Related Target Proteins in Swine Monocytes
    Article Snippet: .. A total of 2 μg RNA from each sample was sent for genome-wide microRNA microarray analysis using μParaflo® microfluidic biochip technology; this service was provided by LC Sciences (Houston, TX, USA). ..

    Article Title: MicroRNA-126 regulates DNA methylation in CD4+ T cells and contributes to systemic lupus erythematosus by targeting DNA methyltransferase 1.
    Article Snippet: .. The pooled samples were sent to LC Sciences for microRNA microarray analysis. .. RNA quality control, labeling, hybridization, and scanning were performed at LC Sciences, using the probes in Sanger miRBase microRNA database version 11.0 (http://www.ebi.ac.uk/).

    Article Title: MicroRNA‐9 enhances migration and invasion through KLF17 in hepatocellular carcinoma
    Article Snippet: .. MicroRNA microarray analysis Microarray assay and data analysis were performed using a service provider (LC Sciences). miRNA profiling was performed by high‐throughput quantitative qRT‐PCR using the TaqMan ® Array Human MicroRNA Cards V3.0 (Applied Biosystems) following the manufacturer's instructions. ..

    Irradiation:

    Article Title: Micro-RNA30c Negatively Regulates REDD1 Expression in Human Hematopoietic and Osteoblast Cells after Gamma-Irradiation
    Article Snippet: RNA concentrations were determined by measuring OD on the NanoDrop spectrophotometer ND-1000 (Thermo Fisher Scientific) and total RNA quality was verified on the Agilent 2100 bioanalyzer (Agilent Technologies) with RNA 6000 Nano chips. .. MicroRNA microarray analysis was performed by LC Sciences (Houston, Texas) on unirradiated or 2 or 8 Gy γ-irradiated human CD34+ and hFOB cells collected 1 h after irradiation. ..

    Article Title: Micro-RNA30c negatively regulates REDD1 expression in human hematopoietic and osteoblast cells after gamma-irradiation.
    Article Snippet: RNA concentrations were determined by measuring OD on the NanoDrop spectrophotometer ND-1000 (Thermo Fisher Scientific) and total RNA quality was verified on the Agilent 2100 bioanalyzer (Agilent Technologies) with RNA 6000 Nano chips. .. MicroRNA microarray analysis was performed by LC Sciences (Houston, Texas) on unirradiated or 2 or 8 Gy c-irradiated human CD34+ and hFOB cells collected 1 h after irradiation. ..

    Expressing:

    Article Title: Mutant p53-R273H gains new function in sustained activation of EGFR signaling via suppressing miR-27a expression
    Article Snippet: H1299-Tet-On-p53 and H1299-Tet-On-p53 273H cells were treated with or without doxycycline for 24 h. Total RNA from these cells was isolated with TRIzol reagent (Invitrogen, Grand Island, NY, USA). .. To compare the miRNA expression profiles between wild-type p53- and mutant p53-expressing H1299 cells, the μParaflo MicroRNA microarray analysis was performed by LC Sciences (Houston, TX, USA). ..

    Mutagenesis:

    Article Title: Mutant p53-R273H gains new function in sustained activation of EGFR signaling via suppressing miR-27a expression
    Article Snippet: H1299-Tet-On-p53 and H1299-Tet-On-p53 273H cells were treated with or without doxycycline for 24 h. Total RNA from these cells was isolated with TRIzol reagent (Invitrogen, Grand Island, NY, USA). .. To compare the miRNA expression profiles between wild-type p53- and mutant p53-expressing H1299 cells, the μParaflo MicroRNA microarray analysis was performed by LC Sciences (Houston, TX, USA). ..

    Genome Wide:

    Article Title: Immunomodulation by Bifidobacterium animalis subsp. lactis Bb12: Integrative Analysis of miRNA Expression and TLR2 Pathway–Related Target Proteins in Swine Monocytes
    Article Snippet: .. A total of 2 μg RNA from each sample was sent for genome-wide microRNA microarray analysis using μParaflo® microfluidic biochip technology; this service was provided by LC Sciences (Houston, TX, USA). ..



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    Flowchart of <t>microarray</t> data analysis. Swine monocytes were exposed to Bb12 with or without the addition of a blocking antibody anti-TLR2. Groups were as follows: unstimulated swine monocytes, monocytes stimulated with Bb12 for 4 h, and swine monocytes incubated with anti-TLR2 antibody and Bb12 for 4 h. Microarray was performed, and 40 microRNAs with a MFI > 500 were selected for analysis of molecular interactions (KEGG analysis). The microRNAs were also analyzed for miRNA–mRNA interactions within TLR2 pathway targets (miRTarBase). After this, 15 miRNAs with a reported interaction with TLR2 pathway–related target proteins were selected to analyze with a multiple t test
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    Image Search Results


    Flowchart of microarray data analysis. Swine monocytes were exposed to Bb12 with or without the addition of a blocking antibody anti-TLR2. Groups were as follows: unstimulated swine monocytes, monocytes stimulated with Bb12 for 4 h, and swine monocytes incubated with anti-TLR2 antibody and Bb12 for 4 h. Microarray was performed, and 40 microRNAs with a MFI > 500 were selected for analysis of molecular interactions (KEGG analysis). The microRNAs were also analyzed for miRNA–mRNA interactions within TLR2 pathway targets (miRTarBase). After this, 15 miRNAs with a reported interaction with TLR2 pathway–related target proteins were selected to analyze with a multiple t test

    Journal: Probiotics and Antimicrobial Proteins

    Article Title: Immunomodulation by Bifidobacterium animalis subsp. lactis Bb12: Integrative Analysis of miRNA Expression and TLR2 Pathway–Related Target Proteins in Swine Monocytes

    doi: 10.1007/s12602-021-09816-1

    Figure Lengend Snippet: Flowchart of microarray data analysis. Swine monocytes were exposed to Bb12 with or without the addition of a blocking antibody anti-TLR2. Groups were as follows: unstimulated swine monocytes, monocytes stimulated with Bb12 for 4 h, and swine monocytes incubated with anti-TLR2 antibody and Bb12 for 4 h. Microarray was performed, and 40 microRNAs with a MFI > 500 were selected for analysis of molecular interactions (KEGG analysis). The microRNAs were also analyzed for miRNA–mRNA interactions within TLR2 pathway targets (miRTarBase). After this, 15 miRNAs with a reported interaction with TLR2 pathway–related target proteins were selected to analyze with a multiple t test

    Article Snippet: A total of 2 μg RNA from each sample was sent for genome-wide microRNA microarray analysis using μParaflo® microfluidic biochip technology; this service was provided by LC Sciences (Houston, TX, USA).

    Techniques: Microarray, Blocking Assay, Incubation

    Comparison of qRT-PCR and microarray results. Log2 fold change expression between unstimulated monocytes and Bb12-stimulated cells by qRT-PCR and microarray

    Journal: Probiotics and Antimicrobial Proteins

    Article Title: Immunomodulation by Bifidobacterium animalis subsp. lactis Bb12: Integrative Analysis of miRNA Expression and TLR2 Pathway–Related Target Proteins in Swine Monocytes

    doi: 10.1007/s12602-021-09816-1

    Figure Lengend Snippet: Comparison of qRT-PCR and microarray results. Log2 fold change expression between unstimulated monocytes and Bb12-stimulated cells by qRT-PCR and microarray

    Article Snippet: A total of 2 μg RNA from each sample was sent for genome-wide microRNA microarray analysis using μParaflo® microfluidic biochip technology; this service was provided by LC Sciences (Houston, TX, USA).

    Techniques: Comparison, Quantitative RT-PCR, Microarray, Expressing

    Mir-92b promoted osteogenesis in MSCs (A–B) The top three up/down-regulated microRNAs in De-Os-MSCs were listed, and verified by qPCR (B) (C–E) The scrambled control, let-7e, mir-10b, mir-20a, mir-92b, mir-371 and mir-373 were transduced into MSCs with lentiviruses. The overexpression of each microRNA was verified by qPCR (C). The infected MSCs were induced to undergo osteogenic differentiation for 10 days, then the calcium deposits were stained with Alizarin Red S (D), and quantified (E) (F) Total RNA was extracted from MSCs infected with mir-92b or scrambled control. The mRNA expression levels of Osterix, Runx2, OPN and ALP were detected by qPCR. β-actin was used as an internal control. The data was expressed as mean ​± ​SD (n ​= ​3). ∗p ​< ​0.05 (G–I) Total proteins were extracted from MSCs transduced with scrambled control or mir-92b. Then the proteins were analyzed by western blot using indicated antibodies. The protein levels of pERK (H) and pJNK (I) was normalized to ERK and JNK1 respectively. All the data represent mean ​± ​SD of three independent experiments. ∗p ​< ​0.05 (J–K) The mir-92b antagmir was transfected into MSCs, then the cells were treated with osteogenic induction medium for 10 days, the calcium deposits were stained with Alizarin Red S (J), the changes of osteogenesis-related genes was checked by qPCR (K).

    Journal: Journal of Orthopaedic Translation

    Article Title: De-osteogenic-differentiated mesenchymal stem cells accelerate fracture healing by mir-92b

    doi: 10.1016/j.jot.2020.10.009

    Figure Lengend Snippet: Mir-92b promoted osteogenesis in MSCs (A–B) The top three up/down-regulated microRNAs in De-Os-MSCs were listed, and verified by qPCR (B) (C–E) The scrambled control, let-7e, mir-10b, mir-20a, mir-92b, mir-371 and mir-373 were transduced into MSCs with lentiviruses. The overexpression of each microRNA was verified by qPCR (C). The infected MSCs were induced to undergo osteogenic differentiation for 10 days, then the calcium deposits were stained with Alizarin Red S (D), and quantified (E) (F) Total RNA was extracted from MSCs infected with mir-92b or scrambled control. The mRNA expression levels of Osterix, Runx2, OPN and ALP were detected by qPCR. β-actin was used as an internal control. The data was expressed as mean ​± ​SD (n ​= ​3). ∗p ​< ​0.05 (G–I) Total proteins were extracted from MSCs transduced with scrambled control or mir-92b. Then the proteins were analyzed by western blot using indicated antibodies. The protein levels of pERK (H) and pJNK (I) was normalized to ERK and JNK1 respectively. All the data represent mean ​± ​SD of three independent experiments. ∗p ​< ​0.05 (J–K) The mir-92b antagmir was transfected into MSCs, then the cells were treated with osteogenic induction medium for 10 days, the calcium deposits were stained with Alizarin Red S (J), the changes of osteogenesis-related genes was checked by qPCR (K).

    Article Snippet: The microRNA microarray analysis was performed by the Annoroad Gene Technology Corporation (Beijing, China).

    Techniques: Control, Over Expression, Infection, Staining, Expressing, Transduction, Western Blot, Transfection

    Validation of candidate miRNAs. Among the top rated nine miRNAs screened from our miRNA microarray, four miRNAs involving (A) hsa-miR-145-5p, (B) hsa-miR-497-5p, (C) hsa-miR-29a-3p and (D) hsa-miR-204-5p were also significantly altered in GSE40355. miRNA, microRNA.

    Journal: Molecular Medicine Reports

    Article Title: Identification and bioinformatics analysis of miRNAs associated with human muscle invasive bladder cancer

    doi: 10.3892/mmr.2017.7726

    Figure Lengend Snippet: Validation of candidate miRNAs. Among the top rated nine miRNAs screened from our miRNA microarray, four miRNAs involving (A) hsa-miR-145-5p, (B) hsa-miR-497-5p, (C) hsa-miR-29a-3p and (D) hsa-miR-204-5p were also significantly altered in GSE40355. miRNA, microRNA.

    Article Snippet: After assessing RNA quality and quantity, the miRNAs microarray analysis (Affymetrix microRNA 4.0 Array, Affymetrix, Inc., Santa Clara, CA, USA) was performed according to the manufacturer's instructions.

    Techniques: Microarray